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pgl3 basic  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic
    Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3-basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm41713257-68-22-27
    Average 93 stars, based on 51 article reviews
    pgl3 basic - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Plasmid Preparation:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control. .. The protein concentrations were ascertained through the Bradford protein assay (Bio-Rad Laboratories, Milano, Italy).

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Construct:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma.
    Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis.

    Clone Assay:

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: GAL3ST1-Mediated Histone Tyrosine Sulfation Induced by Cancer-Associated Fibroblasts Promotes Gastric Cancer Metastasis
    Article Snippet: .. 180 Briefly, GAL3ST1 promoter fragments were amplified and cloned into the pGL3 181 (RRID: Addgene_48743) basic vector. .. Luciferase activity was examined using a Dual 182 Luciferase Assay (Promega, Madison, WI, USA) following the manufacturer’s 183 instructions.

    Control:

    Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma.
    Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis.

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control. .. The protein concentrations were ascertained through the Bradford protein assay (Bio-Rad Laboratories, Milano, Italy).

    Luciferase:

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Reporter Assay:

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Amplification:

    Article Title: GAL3ST1-Mediated Histone Tyrosine Sulfation Induced by Cancer-Associated Fibroblasts Promotes Gastric Cancer Metastasis
    Article Snippet: .. 180 Briefly, GAL3ST1 promoter fragments were amplified and cloned into the pGL3 181 (RRID: Addgene_48743) basic vector. .. Luciferase activity was examined using a Dual 182 Luciferase Assay (Promega, Madison, WI, USA) following the manufacturer’s 183 instructions.



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